neural pathway · histology

🧬 Histology · USMLE Step 1

Connective tissue · cartilage · bone · muscle · nervous · blood · glands
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🧭 Four basic tissues

  • Epithelium – avascular, minimal ECM, covers/lines, secretion
  • Connective tissue – abundant ECM, vascular (most), support/defense
  • Muscle – contraction (skeletal, cardiac, smooth)
  • Nervous – signal conduction (neurons + glia)
🔬 key Epithelium: no blood vessels; CT: defined by ECM & ground substance.

🧶 ECM · fibers

  • Collagen I – bone, tendon, ligament, dermis (tensile)
  • Collagen II – hyaline & elastic cartilage
  • Collagen III (reticular) – lymphoid organs, liver, marrow
  • Collagen IV – basement membrane (non‑fibrillar)
  • Elastic – elastin + fibrillin; large arteries, lung
⚠️ Scurvy (Vit C def) → impaired hydroxylation of collagen. Osteogenesis imperfecta → type I collagen defect (blue sclerae, brittle bones).

🧫 CT cells

  • Fibroblast – produces collagen, GAGs (main resident)
  • Macrophage – phagocytosis, antigen presentation
  • Mast cell – histamine/heparin; IgE‑mediated degranulation
  • Plasma cell – antibody secretion; clock‑face nucleus
  • Adipocyte – white (storage) / brown (thermogenesis, UCP‑1)
mast cell tissue‑resident; basophil = circulating counterpart.

📦 CT proper subtypes

  • Loose (areolar) – beneath epithelia, around vessels
  • Dense regular – tendons, ligaments (parallel collagen)
  • Dense irregular – dermis, capsules (multidirectional)
  • Reticular – lymph nodes, spleen, marrow (type III)
  • Adipose white – unilocular, energy storage
  • Adipose brown – multilocular, UCP‑1, thermogenesis
  • Mesenchyme – embryonic, gives rise to all CT
  • Mucous (Wharton jelly) – umbilical cord

🦴 Cartilage

  • Hyaline – type II collagen; articular, trachea, nose (glassy)
  • Elastic – type II + elastic fibers; ear, epiglottis
  • Fibrocartilage – type I + II; IV discs, pubic symphysis
  • Perichondrium – absent in articular & fibrocartilage
  • Growth: appositional (perichondrium) + interstitial (chondrocyte division)
⚠️ Achondroplasia = FGFR3 gain‑of‑function → impaired endochondral growth (most common dwarfism).

🏗️ Bone

  • Osteoblast – synthesizes osteoid (type I collagen); becomes osteocyte
  • Osteoclast – multinucleated, resorption (H⁺ ATPase, acid hydrolases)
  • Compact – osteons (Haversian systems), Volkmann canals
  • Spongy – trabeculae, marrow spaces, higher surface area
  • Intramembranous – skull, clavicle (no cartilage model)
  • Endochondral – long bones, growth plate (cartilage model)
osteoporosis osteoclast > osteoblast (postmenopausal)  ·  osteopetrosis defective osteoclast (marble bone)

🩸 Blood & hematopoiesis

  • RBC – anucleate, biconcave, 120d lifespan
  • Neutrophil – multilobed, acute bacterial infection
  • Eosinophil – bilobed, bright granules; allergy, parasite
  • Basophil – histamine/heparin; type I hypersensitivity
  • Lymphocyte – B/T/NK, adaptive immunity
  • Monocyte – kidney‑shaped nucleus → macrophage
  • Platelet – megakaryocyte fragment, hemostasis
hematopoiesis yolk sac (3‑8w) → liver/spleen (fetal) → bone marrow (postnatal). Extramedullary in marrow failure (myelofibrosis, thalassemia).

💪 Muscle tissue

  • Skeletal – striated, multinucleated, voluntary, satellite cells (limited repair)
  • Cardiac – striated, single central nucleus, intercalated discs, involuntary
  • Smooth – non‑striated, single nucleus, involuntary, good regeneration
sarcomere A band (myosin, constant), I band (actin, shortens), H zone (myosin only, shortens), Z line (α‑actinin), M line (myosin anchor)
⚠️ Duchenne – dystrophin (X‑linked), pseudohypertrophy calves. Myasthenia gravis – anti‑AChR Ab, fatigable weakness.

🧠 Nervous tissue

neuron
  • Dendrites – receive input
  • Axon – conducts AP; hillock = trigger zone
  • Nissl bodies = RER (protein synthesis)
glia
  • Astrocyte – BBB, GFAP, K⁺ buffering
  • Oligodendrocyte – CNS myelin (multiple axons)
  • Schwann cell – PNS myelin (single axon)
  • Microglia – CNS macrophage
  • Ependymal – CSF production
MS CNS demyelination (oligodendrocyte)  ·  GBS PNS demyelination (Schwann cell)

🧴 Glands

  • Exocrine – duct to surface (salivary, pancreas acinar)
  • Endocrine – ductless → hormone to blood (thyroid, adrenal)
  • Merocrine – exocytosis (salivary, eccrine)
  • Apocrine – apical pinch‑off (mammary, axillary)
  • Holocrine – entire cell disintegrates (sebaceous)

⚠️ Clinical pearls · USMLE

  • Scurvy – Vit C → collagen hydroxylation
  • OI – type I collagen (blue sclerae)
  • Achondroplasia – FGFR3 (endochondral)
  • Osteoporosis – osteoclast > osteoblast
  • Osteopetrosis – defective osteoclast
  • Duchenne – dystrophin X‑linked
  • MS – CNS oligodendrocyte
  • GBS – PNS Schwann cell
  • Paget – mosaic cement lines
  • Ehlers‑Danlos – collagen cross‑linking defect
🔬 framework Collagen synthesis defect · matrix mineralization defect · cell activity defect (osteoblast/osteoclast/chondrocyte)